Mechanoactivation of Enzymes by Single Molecule Force Spectroscopy Hermann Gaub, University of Munich, Germany
Session I: New AFM-based instrumentation for biology and medicine Daniel Fletcher, University of California, Berkeley, USA
8:55 Chair Introduction
9:00 Ultrastable atomic force microscopy: atomic-scale lateral stability and registration in ambient conditions Tom Perkins, University of Colorado, USA
9:30 Mapping stiffness and surface charge density of membrane proteins with microsecond force spectroscopy Ozgur Sahin, Harvard University, Cambridge, Massachusetts, USA
9:50 Micromachined membrane probes for parallel single molecule force spectroscopy Hamdi Torun, K K Sarangapani, O Finkler, F L Degertekin, Georgia Institute of Technology, Atlanta, Georgia, USA
10:10 am Break
10:50 Lateral fusion of lipid membranes to nanoscale functionalized posts Nick Melosh, Stanford University, USA
11:20 Quantitative dynamic force spectroscopy with miniature cantilevers: taking the direct measurement of molecular interactions two steps further Allard J. Katan and Tjerk H. Oosterkamp, Leiden University, Leiden, The Netherlands
11:40 TBD Tip enhanced Raman spectroscopy for label-free biomolecules detection Minhua Zhao, Vu Quang, Albert J. Jin, Paul D. Smith, NIBIB, NIH, Bethesda, Maryland, USA
12:00 AFM Probes with Selectively Attached and Oriented Ultra-High Aspect Ratio Metal Nanoneedles and Their Applicability to in situ Measurements and Manipulations of Soft Biomaterials Mehdi M. Yazdanapanah, Mahdi Hosseini, Santosh Pabba, Brigitte H. Fasciotto, Scott M. Berry, Vladimir V. Dobrokhotov, Abdelilah Safir, Robert S. Keynton and Robert W. Cohn, University of Louisville, Louisville, Kentucky, USA
12:20 Modified AFMs for cell mechanical measurements Daniel Fletcher, University of California, Berkeley, USA
12:50 Poster Session I (Lunch Provided)
2:45 Chair Introduction
Session II: Biomolecular Force Spectroscopy Christopher Yip, University of Toronto, Canada
2:50 Characterizing interactions that modulate the functional state of single membrane proteins Daniel Mueller, Biotechnology Center, TU Dresden, Germany
3:20 Prospects and perils of single-molecule force-spectroscopy on live cells Volkmar Heinrich, University of California at Davis, California, USA
3:40 Nebulin elasticity and unfolding of transient a-helices: a stretched protein ruler pre-loads thin filaments of the skeletal muscle Jeffrey G. Forbes, Vamsi K. Yadavali, Wanxia L. Tsai, and Kuan Wang, Virginia Commonwealth University, Richmond, Virginia
4:00 Distinct kinetic and mechanical properties govern ALCAM-mediated interactions as shown by single-molecule force spectroscopy Joost te Riet, Alessandra Cambi, Sylvia Speller, Frank de Lange and Carl G. Figdor, Radboud University Nijmegen, The Netherlands
4:20 pm Break
4:50 A novel functional single-molecule binding assay via force spectroscopy Hongbin Li, University of British Columbia, Canada
5:20 Multiscale stochastic dynamical models calibrated from single-molecule force spectroscopy time series Christopher P. Calderon, Wei-Hung Chen, Nolan Harris, Kuan-Jiuh Lin, Dennis D. Cox and Ching-Hwa Kiang Rice University, Houston, Texas USA
5:40 Force recognition localization of specific membrane proteins on the primary cilia on MDCK cells J.E. Evangelides, D.N. Sheppard, Terry J. McMaster, University of Bristol, U.K
6:00 Casting a Wide Net: Applications and Challenges in Force Spectroscopy Christopher Yip, University of Toronto, Canada
6:30pm Break
7:30 Selected Best Short Presentations/Ten "6 minutes slots presentations"
8:30 End
Friday, October 17
8:25 Chair Introduction
Session III: AFM of Biomaterial Surfaces Greg Haugstad, University of Minnesota, USA
8:30 Exploring Protein-Surface Interactions in Biomaterials Science by Atomic Force Microscopy Chris Siedlecki, Pennsylvania State University, USA
9:00 Design and AFM characterization of electrospun polymer nanofibers and collagen membranes for regenerative medicine, 3D cancer cell culture models and drug screening applications Olga Hartman, Chu Zhang, Elizabeth Adams, Cindy M. Farach-Carson, Cheryl Casper, Bruce Chase and John Rabolt, University of Delaware, Newark, USA
9:20 Immobilizing Cecropin P1 for Detection of Pathogenic E. coli Joshua Strauss, Charlene Mello, Terri A. Camesano, Worcester Polytechnic Institute MA, USA
9:40 TBD Phase changes in supported planar bilayers of a lipochaperone Laura Picas, M. Teresa Montero, Antoni Morros, Gerard Oncins, Jordi Hernández-Borrell, University of Barcelona Spain
10am Break
10:30 Nanoscopy: Novel Concepts and Application to Biomedicine Harald Fuchs, University of Münster, Germany
11:00 An atomic force microscopy investigation of the interfacial properties of biocompatible nanostructured Titanium Oxide films V.Vyas, S. Bovio, Alessandro Podestà, G. Bongiorno, P. Piseri, P. Milani Universita' degli Studi di Milano, Milano, Italy
11:20 Microscopic analyses of bone mineral dissolution Seung-Wuk Lee, Ki-Young Kwon, Eddie Wang, Alice Chung, Neil Chang, University of California, Berkeley, Ca, USA
11:40 Environmental Digital Pulsed Force Mode AFM in Biomedical Coatings Research Greg Haugstad, University of Minnesota, USA
12:10 Poster Session II (Lunch Provided)
2:05 Chair Introduction
Session IV: AFM of Cells Michel Grandbois, Université de Sherbrooke, Canada
2:10 AFM of Biomaterial Surfaces Jan Hoh, John Hopkins University, Maryland USA
2:40 Imaging the Cellular Cytoskeleton with Atomic Force Microscopy Helen McNally, Gina-Mirela Mustata,Ken Ritchie, Purdue University, Indiana, USA
3:00 Probing molecular and cellular mechanobiology in the nervous system Sanjay Kumar UC Berkeley, California, USA
3:20 Mechanically induced membrane potential change of living endothelial cells observed in real time by combined AFM-fluorescence microscopy C. Callies, Peter Schön, I. Liashkovich, H. Oberleithner, University of Münster, Germany
3:40 pm Break
4:10 Investigation of cellular Mechanics by AFM Manfred Radmacher, University of Bremen, Germany
4:40 Active and passive biomechanics of single cells and cell aggregates Anatol Fritsch, Karla Mueller, Franziska Wetzel, Claudia Bruner, Michael Goegler, Josef Käs University of Leipzig, Germany
5:00 Cellular Microbiology and Infectious Pathogeny Sébastien Janel, Frank Lafont, Pasteur Institute Lille, France
5:20 Morphology and Biomechanics of Live Neuronal Growth Cones Ying Xiong, Aih Cheun Lee, Daniel M. Suter and Gil U Lee, Purdue University
5:40 AFM-Based Force Sensing Applied to Cell Biology and Potential Applications in Drug Screening Michel Grandbois, Université de Sherbrooke, Canada
Friday 7:00-9:00 pm
Gala Dinner
Monterey Bay Aquarium
8:30 Presentation of Poster Award
Saturday, October 18
8:25 Chair Introduction
Session V: Biomolecular Imaging Simon Scheuring, Institut Curie, France
8:30 Protein Misfolding and Aggregation: Probing with the AFM force Spectroscopy Yuri Lyubchenko, University of Nebraska, USA
9:00 Biological processes on a membrane observed by atomic force microscopy Tomoji Kawai, A. Takagi, H. Hokonohara, E. Mikamo-Satoh, and T. Kobayashi Osaka University, Japan
9:20 AFM-Assembly: Atomic force microscopy and structural biology Jean-Luc Pellequer CEA Marcoule France
9:40 Lighting up amyloid-beta oligomers: effects of fluorophore conjugation on assembly and function Lisa M. Jungbauer, C. Yu, Mary Jo LaDu, University of Illinois at Chicago, Illinois, USA
10 am Break
10:30 Insight into the pore-forming process of a bacterial toxin from atomic force microscopy Zhifeng Shao, University of Virginia, USA
11:00 Remodeling of amyloid fibrils by the polyphenol EGCG monitored in situ by AFM Jenny Russ, Dagmar Ehrnhoefer, Erich Wanker, Jan Bieschke, Max Delbrueck Center, Berlin-Buch, Germany
11:20 High Resolution Bimolecular Imaging and Mechanical Mapping in Fluid Nelly Traitcheva, Yan Hu and Chanmin Su, Veeco Instruments, Santa Barbara, California, USA
11:40 High-resolution AFM in biological research and as a medical nano-imaging tool Simon Scheuring, Institut Curie, France
